Post-transcriptional gene regulation by an Hfq-independent small RNA in Caulobacter crescentus

An RNA degradosome assembly in Caulobacter crescentus

In many bacterial species, the multi-enzyme RNA degradosome assembly makes key contributions to RNA metabolism. Powering the turnover of RNA and the processing of structural precursors, the RNA degradosome has differential activities on a spectrum of transcripts and contributes to gene regulation at a global level. Here, we report the isolation and characterization of an RNA degradosome assembl...

Spectroscopic observation of RNA chaperone activities of Hfq in post-transcriptional regulation by a small non-coding RNA

Hfq protein is vital for the function of many non-coding small (s)RNAs in bacteria but the mechanism by which Hfq facilitates the function of sRNA is still debated. We developed a fluorescence resonance energy transfer assay to probe how Hfq modulates the interaction between a sRNA, DsrA, and its regulatory target mRNA, rpoS. The relevant RNA fragments were labelled so that changes in intra- an...

Caulobacter crescentus both transcriptional activation and negative

Regulation of cellular differentiation in Caulobacter crescentus.

In Caulobacter crescentus, asymmetry is generated in the predivisional cell, resulting in the formation of two distinct cell types upon cell division: a motile swarmer cell and a sessile stalked cell. These progeny cell types differ in their relative programs of gene expression and DNA replication. In progeny swarmer cells, DNA replication is silenced for a defined period, but stalked cells rei...

Post-Transcriptional Gene Regulation by MicroRNA and RNA-Binding Protein

Recent advanced studies have demonstrated that post-transcriptional gene regulation is involved in many aspects of biological processes and in the pathogenesis of various types of disorders, such as neurodegenerative diseases, autoimmune diseases, and cancer. In addition to transcriptional regulation, spatially and temporally regulated gene expression is achieved by the post-transcriptional con...